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本文介绍分离霍乱弧菌缺失突变株的方法,用已知特异性的限制核酸内切酶消化,预先确定缺失.通过在携带具有邻近侧翼顺序的所需缺失的质粒与霍乱弧菌染色体之间进行体内重组,把该缺失插入霍乱弧菌染色体中.本文还介绍了具有毒性
This article describes a method for isolating Vibrio cholera deletion mutants that have been pre-determined for deletion by digestion with known specific restriction endonucleases. By carrying out the process between plasmids harboring the desired deletion with the adjacent flanking sequences and V. cholerae chromosomes In vivo recombination, the insertion of this deletion into the V. cholerae chromosome. This article also describes the toxic