论文部分内容阅读
AIM:Hepatitis B virus(HBV)genomes in carriers fromHawaii have not been evaluated previously.The aim of thepresent study was to evaluate the distribution of HBVgenotypes and their clinical relevance in Hawaii.METHODS:Genotyping of HBV among 61 multi-ethniccarriers in Hawaii was performed by genetic methods.Three complete genomes and 61 core promoter/precoreregions of HBV were sequenced directly.RESULTS:HBV genotype distribution among the 61 carrierswas 23.0% for genotype A,14.7% for genotype B and 62.3%for genotype C.Genotypes A,B and C were obtained fromthe carriers whose ethnicities were Filipino and Caucasian,Southeast Asian,and various Asian and Micronesian,respectively.All cases of genotype B were composed ofrecombinant strains with genotype C in the precore pluscore region named genotype Ba.HBeAg was detected morefrequently in genotype C than in genotype B(68.4% vs33.3%,P<0.05)and basal core promoter(BCP)mutation(T1762/A1764)was more frequently found in genotype Cthan in genotype B.Twelve of the 38 genotype C strainspossessed C at nucleotide(nt)position 1858(C-1858).However there was no significant difference in clinicalcharacteristics between C-1858 and T-1858 variants.Basedon complete genome sequences,phylogenetic analysisrevealed one patient of Micronesian ethnicity as having C-1858 clustered with two isolates from Polynesia with T-1858.In addition,two strains from Asian ethnicities were clusteredwith known isolates in carriers from Southeast Asia.CONCLUSION:Genotypes A,B and C are predominanttypes among multi-ethnic HBV carriers in Hawaii,anddistribution of HBV genotypes is dependent on the ethnicbackground of the carriers in Hawaii.
AIM: Hepatitis B virus (HBV) genomes in carriers from Hawaii have not been omitted previously. The aim of the present study was to evaluate the distribution of HBV genotypes and their clinical relevance in Hawaii. METHODS: Genotyping of HBV among 61 multi-ethnic carriers in Hawaii was performed by genetic methods. Whole genomes and 61 core promoter / precoreregions of HBV were sequenced directly .RESULTS: HBV genotype distribution among the 61 carrierswas 23.0% for genotype A, 14.7% for genotype B and 62.3% for genotype C. Genotypes A, B and C were obtained from the carriers whose ethnicities were Filipino and Caucasian, Southeast Asian, and various Asian and Micronesian, respectively. All cases of genotype B were composed of recombinant strains with genotype C in the precore pluscore region named genotype Ba. HBeAg was detected more frequently in genotype C than in genotype B (68.4% vs33.3%, P <0.05) and basal core promoter (BCP) mutation (T1762 / A1764) was more frequently found in genotype Cthan in genotype B. There was no significant difference in clinical characteristics between C-1858 and T-1858 variants. Based on complete genome sequences, phylogenetic analysis was one of the patients of Micronesian ethnicity having C-1858 clustered with two isolates from Polynesia with T-1858.In addition, two strains from Asian ethnicities were clusteredwith known isolates in carriers from Southeast Asia.CONCLUSION: Genotypes A, B and C are predominanttypes among multi- ethnic HBV carriers in Hawaii, and of of HBV genotypes is dependent on the ethnic background of the carriers in Hawaii.