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目的探讨细胞免疫功能及辅助型T细胞(Th)细胞因子在口腔扁平苔藓(OLP)中的作用。方法以35例糜烂型(糜烂组)及29例网纹型OLP(网纹组)患者为对象,选取同期20例健康人为对照组,流式细胞术检测外周血中T细胞、B细胞、NK细胞和调节性T细胞亚群表达百分率。ELISA法检测外周血中TNF-α和INF-γ(Th1型细胞因子),IL-4和IL-10(Th2型细胞因子),IL-17、IL-23(Th17型细胞因子)含量;分离外周血单个核细胞,qRT-PCR检测上述因子mRNA的表达。结果与健康组相比,OLP患者CD3~+、CD4~+T细胞亚群比例(F=3.211和3.565,P<0.05)及CD4~+/CD8~+降低(F=3.430,P<0.05),CD4~+Foxp3~+调节性T细胞亚群比例(F=3.370,P<0.05)及外周血中TNF-α、INF-γ、IL-4、IL-10、IL-17、IL-23含量增高(F=0.923、0.820、1.043、1.132、0.745和0.802,P<0.05)。与网纹组相比,糜烂组CD8~+T细胞亚群比例较低(t=2.450,P<0.05);IL-4、IL-10蛋白(t=22.780和25.112,P<0.05)及mRNA较低(t=3.781和6.710,P<0.05),IL-17、IL-23蛋白(t=15.765和19.307,P<0.05)及mRNA较高(t=4.022和8.569,P<0.05)。结论口腔扁平苔藓患者存在细胞免疫紊乱及Th细胞因子异常,其中CD8~+及Th17型细胞与糜烂型OLP发病有关,Th2型细胞与网纹型OLP发病有关。
Objective To investigate the role of cellular immune function and helper T cell (Th) cytokines in oral lichen planus (OLP). Methods Totally 35 healthy patients with erosive (erosive) and 29 (anterior eyelid) veins were enrolled in this study. Twenty healthy controls were enrolled in this study. Flow cytometry was used to detect T cell, B cell, NK Percentage of cells and regulatory T cell subsets expressed. The contents of TNF-αand INF-γ (Th1 type cytokines), IL-4 and IL-10 (Th2 type cytokines), IL-17 and IL-23 (Th17 type cytokines) in peripheral blood were detected by ELISA. Peripheral blood mononuclear cells, qRT-PCR detection of mRNA expression of these factors. Results Compared with the healthy group, the proportion of CD3 ~ + and CD4 ~ + T cells in OLP patients (F = 3.211 and 3.565, P <0.05) and CD4 + / CD8 + , The proportion of CD4 ~ + Foxp3 ~ + regulatory T cell subsets (F = 3.370, P <0.05) and the levels of TNF-α, INF-γ, IL-4, IL-10, IL-17 and IL-23 in peripheral blood Content increased (F = 0.923,0.820,1.043,1.132,0.745 and 0.802, P <0.05). The percentage of CD8 ~ + T cell subsets in erosive group was lower than that in reticulated group (t = 2.450, P <0.05), IL-4 and IL-10 protein IL-17 and IL-23 (t = 15.765 and 19.307, P <0.05) and mRNA (t = 4.022 and 8.569, P <0.05) were lower (t = 3.781 and 6.710, P <0.05). Conclusion There are cellular immune disorders and abnormalities of Th cytokines in patients with oral lichen planus. Among them, CD8 + and Th17 cells are associated with the pathogenesis of erosive OLP and Th2 cells are associated with the pathogenesis of.