目的：纯化单克隆抗体（ 单抗，ＭｃＡｂ） 。方法：应用Ｐｒｏｔｅｉｎ ＡＳｅｐｈａｒｏｓｅ ＣＬ４Ｂ制备免疫亲和层析柱，纯化载脂蛋白（Ａｐｏ）Ｅ４、ＡｐｏＥ６、抑制素（ｉｎｈｉｂｉｎＩＮＨ）α、βＡ、βＢ５ 种ＭｃＡｂ。结果：ＭｃＡｂ 纯化后电泳呈一条带，并有较好的免疫活性。结论：应用蛋白Ａ亲和层析法可以纯化ＭｃＡｂ。 Objective: To purify monoclonal antibody (McAb, McAb). Methods: Protein A Sepharose CL4B was used to prepare immunoaffinity chromatographic column to purify five McAbs of Apo E4, ApoE6, inhibin INH, βA and βB. Results: McAb purified electrophoresis showed a band, and has good immunocompetence. Conclusion: McAb can be purified by protein A affinity chromatography.