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通过RT-PCR扩增了黄瓜绿斑驳花叶病毒(Cucumber green mottle mosaic virus,CGMMV)济南分离物(CGMMV-JN)的基因组片段。序列测定结果表明CGMMV-JN基因组全长6 424核苷酸(nt),5’-和3’-UTR分别为60和176 nt,含有4个ORF,分别编码129 kDa和186 kDa复制酶相关蛋白、29 kDa移动蛋白及17.4 kDa外壳蛋白。CGMMV-JN与另外29个CGMMV分离物全基因组核苷酸序列一致率为90.0%~99.7%。重组分析发现韩国KOM(AF417243)、以色列EC(KF155231)、印度(DQ767631)和我国河北的CHB(KJ658958)4个分离物在RdRp编码区存在重组。系统发育分析结果表明,这些分离物可分成3个组。选择压力分析结果表明cp基因处于正选择,其它基因处于负选择。本文研究的结果为黄瓜绿斑驳花叶病毒的监测及防控提供了理论依据。
The genomic fragment of CGMMV-JN was amplified by RT-PCR from cucumber green mottle mosaic virus (CGMMV) Jinan isolate. The results of sequence analysis showed that CGMMV-JN genome contained 6 and 6 nt (nt), 5’and 3’-UTR of full length respectively, which contained 4 ORFs encoding the 129 kDa and 186 kDa replicase related proteins , 29 kDa mobile protein and 17.4 kDa coat protein. The identical nucleotide sequence of CGMMV-JN with the other 29 CGMMV isolates was 90.0% ~ 99.7%. Recombinant analysis revealed that 4 isolates from South Korea KOM (AF417243), Israel EC (KF155231), India (DQ767631) and CHB (KJ658958) from Hebei of China existed in the RdRp coding region. Phylogenetic analysis showed that these isolates could be divided into three groups. The results of selection stress analysis showed that cp gene was in positive selection and other genes were negative selection. The results of this study provide a theoretical basis for the monitoring and control of cucumber green mottle mosaic virus.