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采用盆栽及室内模拟实验对铝胁迫下大豆根际微生物的群体感应进行了初步探索。利用高效检测菌株根癌农杆菌Agrobaoterium tumefaciens KYC55(pJZ372;pJZ384;pJZ410)对在酸性土壤中种植了耐铝大豆BX10和铝敏感大豆BD2的根际土、非根际土中群体感应信号分子高丝氨酸酰基内酯(AHL)进行检测。结果表明,在大豆根际土中AHL活性显著高于非根际土,且BX10根际土中AHL含量显著高于BD2(P<0.01);随大豆生长时期的推移,BX10和BD2根际土及非根际土中AHL均逐渐升高。模拟酸铝环境的室内实验表明,不同浓度的铝胁迫对中华根瘤菌Sinorhizobium sp.1128的生长、AHL产生水平、胞外多糖的产生以及根毛吸附作用均产生显著影响,从而进一步验证了铝胁迫对群体感应的影响。
The potted plants and indoor simulation experiments were conducted to explore the population induction of soybean rhizosphere microbes under aluminum stress. In the rhizosphere soils that were resistant to aluminum-tolerant soybean BX10 and aluminum-sensitive soybean BD2 in acidic soils, Agrobacterium tumefaciens KYC55 (pJZ372; pJZ384; pJZ410) Acyl lactones (AHL) were tested. The results showed that the AHL activity in soybean rhizosphere soil was significantly higher than that in non-rhizosphere soil, and the content of AHL in BX10 rhizosphere soil was significantly higher than that in BD2 (P <0.01). With the growth of soybean, BX10 and BD2 rhizosphere soil And non-rhizosphere soil AHL are gradually increased. Laboratory experiments simulating the acid-aluminum environment showed that aluminum stress at different concentrations had significant effects on the growth of Sinorhizobium sp.1128, the level of AHL production, the production of extracellular polysaccharides and the adsorption of root hair, thus further verifying the effect of aluminum stress The impact of group sensing.