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以仙客来干种子为试材,利用“神舟八号”飞船搭载进行太空诱变处理,以此探究太空环境对仙客来造成的诱变效应。结果表明:太空诱变产生的M1代仙客来植株在花色和花形上与对照株相比表现出明显的表观性状差异;对突变株进行的RAPD(Random amplified polymorphic DNA)试验分析中,28条随机引物中有27条引物扩增出现多态性,总共扩增出156条条带,其中91条呈现出多态性,多态性比率达到58.33%。所有突变株的DNA分子均发生了显著变化,变异率从23.08%到33.97%不等。综合分析显示,太空环境对当代植株造成了显著的诱变效应,经辐照后的植株在分子水平上均发生了明显的变异,而基因组的改变进一步导致了植株表观性状的变异。
Cyclamen dry seeds as the test material, the use of “Shenzhou VIII” spacecraft carrying space mutagenesis treatment, in order to explore the space environment on the cyclamen mutagenic effect. The results showed that M1 generation cyclamen plants produced by space mutagenesis showed obvious difference in appearance and flower shape compared with the control plants. In random amplified polymorphic DNA (RAPD) analysis of mutants, A total of 27 random primers were amplified with a total of 156 bands, of which 91 showed polymorphism with a polymorphism rate of 58.33%. All the mutant strains of DNA molecules have undergone significant changes, the mutation rate ranging from 23.08% to 33.97%. Comprehensive analysis showed that the space environment caused significant mutagenic effects on contemporary plants, and the irradiated plants showed obvious variation at the molecular level, while the change of genome further led to the variation of plant apparent traits.