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目的:建立清肺抑火口腔喷雾剂中栀子苷和黄芩苷含量的测定方法。方法:采用HPLC法,以依利特C18柱(4.6mm×200mm,5μm)分离,以乙腈-水(11∶89)和甲醇-水-磷酸(45∶55∶0.2)为流动相,238nm和280nm为检测波长,流速1.0mL.min-1,柱温30℃。结果:栀子苷和黄芩苷分别在1.19~3.95μg(r=0.9998)和1.61~5.63μg(r=0.9998)范围内呈良好的线性关系,平均回收率分别为100.62%(RSD=1.70%)和100.32%(RSD=1.58%)。结论:该方法简便、快速、灵敏度高,可作为清肺抑火口腔喷雾剂中栀子苷和黄芩苷含量的测定方法。
Objective: To establish a method for the determination of geniposide and baicalin in Qingfeiyu Oral Spray. Methods: The HPLC method was used to separate eelite C18 column (4.6 mm × 200 mm, 5 μm) with acetonitrile-water (11:89) and methanol-water-phosphoric acid (45:55:0.2) For the detection wavelength, flow rate 1.0mL.min-1, column temperature 30 ℃. Results: Geniposide and baicalin showed a good linear relationship in the range of 1.19-3.95μg (r = 0.9998) and 1.61-5.63μg (r = 0.9998), with the average recoveries of 100.62% (RSD = 1.70%) And 100.32% (RSD = 1.58%). Conclusion: The method is simple, rapid and sensitive. It can be used as a method for determination of geniposide and baicalin in Qingfeihuoke oral spray.