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目的了解人工游泳池水中致病菌的污染情况。方法于2013—2014年,采集平阳县全部15家正常营业人工游泳池水样进行细菌总数、大肠菌群及致病菌的检测。分离的致病菌进行系统鉴定,并对部分致病菌进行药敏试验、毒力检测。结果共检测250件水样,检出致病菌75株,检出率为30.0%;铜绿假单胞菌的检出率最高(7.6%),沃氏葡萄球菌检出率为5.6%,金黄色葡萄球菌检出率为3.6%。分离的金黄色葡萄球菌(9株)、凝固酶阴性葡萄球菌(23株)对万古霉素、利福平均敏感,其次对青霉素G敏感率最低(分别为22.2%,13.0%),对红霉素、四环素等有不同程度耐药;检出的铜绿假单胞菌(19株)对临床上常用的抗生素未发现耐药情况。金黄色葡萄球菌的肠毒素阳性率为77.8%(7/9);嗜水气单胞菌的脱脂奶平板试验阳性率为80%(4/5)。结论该县人工游泳池水中存在致病菌,且有较高的污染率,部分致病菌带有毒力,对游泳者健康有一定程度的威胁。
Objective To understand the pollution of pathogens in artificial swimming pool water. Methods From 2013 to 2014, all 15 normal swimming pools in Pingyang County were collected for total bacterial count, coliform bacteria and pathogenic bacteria. Isolated pathogenic bacteria were systematically identified, and some of the pathogenic bacteria susceptibility testing, virulence testing. Results A total of 250 water samples were detected and 75 pathogenic bacteria were detected, the detection rate was 30.0%; the highest detection rate of Pseudomonas aeruginosa (7.6%), the detection rate of Staphylococcus wolsens was 5.6% The detection rate of Staphylococcus aureus was 3.6%. Staphylococcus aureus (9 strains) and coagulase - negative staphylococci (23 strains) were sensitive to vancomycin and rifampin, followed by the lowest sensitivity to penicillin G (22.2% and 13.0%, respectively) Su, tetracycline and so have different degrees of resistance; detected Pseudomonas aeruginosa (19 strains) of clinically used antibiotics are not found resistance. Staphylococcus aureus enterotoxin positive rate was 77.8% (7/9); Aeromonas hydrophila flat test positive rate of 80% (4/5). Conclusion There are pathogenic bacteria in the artificial swimming pool water of this county, and have a higher pollution rate. Some pathogenic bacteria are virulent and have a certain threat to the swimmer’s health.