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假孕大鼠口服醋酸棉酚40和80 mg/kg连续5天,平均每个子宫的胞浆蛋白和雌激素受体量明显减少。以每mg蛋白计,雌激素受体浓度同对照组差异不显著。幼大鼠口服醋酸棉酚40mg/kg连续8天,作饱和分析测得对照组和给药组K_d值分别为1.04×10~_(-10)和1.09×10_(-10)mol。最大受体结合数分别为432.0和358.8 fmol/子宫。实验发现棉酚在高浓度时对~3H-雌二醇同其受体的结合有抑制作用。15甲基PGF_(2α)甲酯对假孕大鼠子宫胞浆蛋白水平和雌激素受体均无明显影响。
Oral administration of gossypol acetate 40 and 80 mg / kg for 5 consecutive days to the pseudopregnant rats resulted in a significant decrease in the amount of cytoplasmic protein and estrogen receptors per uterus. The concentration of estrogen receptor per mg protein was not significantly different from the control group. The rats were orally administered with gossypol acetate 40mg / kg for 8 consecutive days. The K_d values of the control group and the administration group were 1.04 × 10 ~ (-10) and 1.09 × 10 ~ (-10) mol, respectively. The maximum number of receptor binding was 432.0 and 358.8 fmol / uterus, respectively. Gossypol was found to inhibit ~ 3H-estradiol binding to its receptor at high concentrations. Methyl PGF_ (2α) methyl ester had no significant effect on uterine cytoplasmic protein and estrogen receptor in pseudopregnant rats.