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目的建立高效液相色谱法测定复方醋酸地塞米松搽剂中地塞米松、樟脑的含量。方法 CAPCELL PAK C18色谱柱(4.6 mm×150 mm,5μm),流动相:乙腈-水(65∶35,V/V),柱温:30℃,流速:1.0 mL.min-1,检测波长:298 nm。结果地塞米松、樟脑进样量分别在0.375～6.00μg(r=0.999 9)、9.69～155.04μg(r=0.999 9)范围内有良好线性关系,平均回收率分别为(100.75±0.83)%、(99.61±0.71)%,RSD分别为0.81%、0.69%(n=9)。结论本方法简便、准确、重复性好,可用于复方醋酸地塞米松搽剂的质量控制。
Objective To establish a HPLC method for the determination of dexamethasone and camphor in compound dexamethasone acetate liniment. Methods CAPCELL PAK C18 column (4.6 mm × 150 mm, 5 μm) was used. The mobile phase was acetonitrile-water (65:35, v / v) 298 nm. Results There was a good linear relationship between dexamethasone and camphor injection in the range of 0.375-6.00μg (r = 0.999 9) and 9.69-155.04μg (r = 0.999 9), with the average recoveries of (100.75 ± 0.83)% , (99.61 ± 0.71)%, RSD 0.81% and 0.69% (n = 9) respectively. Conclusion This method is simple, accurate, reproducible, and can be used for quality control of compound dexamethasone acetate liniment.