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作为拟南芥AtWOX11的同源基因,杨树PeWOX11a和PeWOX11b基因在不定根发生及形态建成过程中发挥重要重要.过表达的PeWOX11a和PeWOX11b转基因杨树不仅不定根数量显著增加,而且在茎和叶上产生大量异位根;同时,两者的过表达也影响转基因杨树腋芽和叶片的发育.为了深入探究两者之间的时空表达调控模式及其生物学功能,本研究克隆和鉴定了PeWOX11a和PeWO11b基因的启动子序列,1 953 bp长度的ProWOX11a和1 772 bp长度的ProWOX11b.两个基因的启动子序列均含有多个植物激素的应答元件或结合位点.启动子GUS表达载体构建及遗传转化分析表明,ProWOX11a启动子驱动GUS基因在根原基启动及随后发育早期的分生组织中特异表达,而ProWOX11b启动子驱动GUS基因在不定根发生的重要区域下胚轴特异表达.“,”As the poplar homologs of AtWOX11,PeWOX11a and PeWOX11b perform key functions in adventitious root formation of poplar.Overexpression of either PeWOX11a or PeWOX11b not only increased the number of adventitious roots on the cuttings but also induced ectopic roots in the aerial parts of transgenic poplars.Elevated the expression of PeWOX11a or PeWOX11b in transgenic poplars also disrupted axillary bud and leaf development.In order to investigate the tissue-specific expression patterns of the PeWOX11 promoters,1 953 bp ProWOX11a and 1 772 bp ProWOX11b promoters were cloned and characterized.The two promoter regions contained multiple hormone-responsive elements.A histochemical beta-glucuronidase (GUS) assay indicated that the ProWOX11a promoter was specifically expressed in the root primordium,while the ProWOX11b promoter was predominantly expressed in the hypocotyl oftransgenic Arabidopsis seedlings.