Objective:Currendy,no satisfactory targets for colorectal cancer or markers for immunotherapy and diagnosis and prognosis are available.Immunoglobulin G (IgG) is widely expressed in many cancers,and it promotes cancer progression.This study explored the role of cancer-derived IgG (CIgG) in colorectal cancer.Methods:First,using a monoclonal antibody to CIgG,we examined the expression levels of CIgG in colorectal cancer cell lines by west blot and immunofluorescence analyses and in tissue specimens by immunohistochemistry.Second,the variable region gene was amplified by nested polymerase chain reaction (PCR),and PCR products were sequenced and analyzed.Third,we investigated the effect of CIgG on colorectal cancer cells by cell proliferation,wound healing,migration and invasion assays,and colony formation assay.Fourth,we performed in vivo tumorigenicity experiments to explore the effect of CIgG on tumorigenicity.Finally,we used RNA-seq analysis and co-immunoprecipitation experiments to further clarify possible mechanisms of CIgG.Results:We found that CIgG is widely expressed in colorectal cancer cells,and the overexpression of CIgG indicates significantly poor colorectal cancer prognosis.Furthermore,CIgG knockdown significantly inhibits the proliferation,migration and invasion ability of cells,and tumor growth in vivo.RNA-seq analysis indicated that CIgG knockdown results primarily in changes in expression of apical junction and epithelial-mesenchymal transition-related genes.CIgG may be involved in colorectal cancer invasion and metastasis through interacting with E-cadherin.Conclusions:CIgG is a potential human oncogene in colorectal cancer and that it has potential for application as a novel target in targeted therapy and a marker for prognostic evaluation.