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应用ＥＬＩＳＡ双抗体夹心法分析了不同胎龄脾、胸腺细胞培养上清和血清ｓＩＬ－２Ｒ的变化。结果显示：（１）胎儿脾、胸腺细胞体外在ｒＩＬ－２和抗ＣＤ３刺激下，上清中可产生高水平的ｓＩＬ－２Ｒ。并且随培养时间的延长显著增高（Ｐ＜０．０１）；（２）胎儿脾细胞培养上清ＳＩＬ－２Ｒ明显高于胸腺（Ｐ＜０．０１）；（３）胎儿血清ｓＩＬ－２Ｒ显著高于成人，并随胎龄的增大逐步下降．胎龄与ｓＩＬ－２Ｒ水平呈负相关。结果提示：ｓＩＬ－２Ｒ不仅是胎儿淋巴细胞活化的标志，还可能在淋巴细胞分化、成熟过程中发挥免疫调节作用。胎儿血清ｓＩＬ－２Ｒ增高并随胎龄成熟而下降，推测可能参与胚胎期免疫耐受。
ELISA double antibody sandwich analysis of different gestational age spleen and thymus cell culture supernatant and serum sIL-2R changes. The results showed that: (1) Fetal spleen and thymocyte could produce high level of sIL-2R in supernatant under the stimulation of rIL-2 and anti-CD3 in vitro. (P <0.01); (2) The content of SIL-2R in fetal spleen cell supernatant was significantly higher than that in thymus (P <0.01); (3) The serum sIL-2R was significantly higher In adults, and with the increase of gestational age and gradually decreased. The gestational age and sIL-2R levels were negatively correlated. The results suggest that: sIL-2R is not only a marker of fetal lymphocyte activation, but also may play an immunomodulatory role in lymphocyte differentiation and maturation. Fetal serum sIL-2R increased and decreased with the mature gestational age, suggesting that may be involved in embryonic immune tolerance.