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本文报道25种甾族激素(雌激素、孕激素、雄激素及类皮质激素等)用反相薄层层析法分离的结果。作者选用三种化学键合硅烷化硅胶(二甲基、辛基或十八烷基硅烷化硅胶)作固定相及5种含水的有机溶剂作移动相。以二甲基硅烷化硅胶作固定相时,用甲醇-水(1:1)及(2:1)作移动相的分离效果较用含等体积水的二氧六环、丙酮、四氢吡喃或乙腈为佳。于紫外254nm处检出的灵敏度为50～100ng/斑点,对雌激素则为1μg/斑
This article reports the results of the separation of 25 steroidal hormones (estrogen, progestin, androgens and corticoids, etc.) by reverse-phase thin-layer chromatography. The authors selected three kinds of chemically bonded silanized silica gel (dimethyl, octyl or octadecylsilanized silica gel) as the stationary phase and five kinds of aqueous organic solvents as the mobile phase. When dimethylsilylation silica was used as the stationary phase, the mobile phase separation with methanol-water (1: 1) and (2: 1) was more efficient than that with mobile phase consisting of water with equal volume of dioxane, acetone and tetrahydropyridine It is preferred that the furan or acetonitrile. The sensitivity at UV 254 nm was 50-100 ng / spot and for estrogen it was 1 μg / spot