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目的研究海洋来源真菌Hypocrea virens发酵液的化学成分。方法采用硅胶柱色谱、Sepha-dex LH-20凝胶柱色谱、开放ODS柱色谱以及HPLC等方法进行分离纯化,通过理化性质和波谱数据鉴定化合物的结构。结果分离得到10个化合物,分别鉴定为bisdethiobis-(methylthio)glio-toxin(1)、胶霉毒素(gliotoxin,2)、5-羟基-3-羟甲基-2-甲基-7-甲氧基色原酮(5-hydroxy-3-hydroxymethyl-2-methyl-7-methoxychromone,3)、6-甲基苯-1,2,4-三醇(6-methyl-benzene-1,2,4-triol,4)、3β-羟基-胆甾-5-烯(3β-hydroxy-cholesta-5-ene,5)、3-异丁基-8-羟基吡咯并哌嗪-2,5-二酮(3-isobutyl-8-hydroxypyrrolopiperazine-2,5-dione,6)、3-苄基哌嗪-2,5-二酮(3-benzylpiperazine-2,5-dione,7)、3-苄基-8-羟基吡咯并哌嗪-2,5-二酮(3-benzyl-8-hydroxypyrrolopiper-azine-2,5-dione,8)、3S*,4R*-二羟基-3-甲基戊烷-2-酮(3S*,4R*-dihydroxy-3-methylpentan-2-one,9)、3R*,4R*-二羟基-3-甲基戊烷-2-酮(3R*,4R*-dihydroxy-3-methylpentan-2-one,10)。结论化合物1、3、4、8～10均为首次从Hypocrea属真菌中分离得到,化合物5为首次从真菌Hypocrea virens中分离得到。
Objective To study the chemical constituents of the marine fermentation fungus Hypocrea virens fermentation broth. Methods The compounds were separated and purified by silica gel column, Sephadex LH-20 column, ODS column and HPLC. The structures of the compounds were identified by physico-chemical properties and spectral data. Results Ten compounds were isolated and identified as bisdethiobis- (methylthio) glio-toxin (1), gliotoxin (2), 5-hydroxy-3-hydroxymethyl-2-methyl- 5-hydroxy-3-hydroxymethyl-2-methyl-7-methoxychromone (3), 6-methyl- triol, 4), 3β-hydroxy-cholesta-5-ene (5), 3- isobutyl-8-hydroxypyrrolopiperazine-2,5-dione 3-benzylpiperazine-2,5-dione (7), 3-benzyl-8-hydroxypyrrolopiperazine- 3-benzyl-8-hydroxypyrrolopiper-azine-2,5-dione (8), 3S *, 4R * -dihydroxy-3-methylpentan-2 3R *, 4R * -dihydroxy-3-methylpentan-2-one (3R *, 4R * -dihydroxy-3-methylpentan- 3-methylpentan-2-one, 10). Conclusion Compounds 1, 3, 4, 8-10 are isolated from fungi of Hypocrea genus for the first time. Compound 5 is isolated from fungi Hypocrea virens for the first time.