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本文报道氯胍、环氯胍、对-氯苯基二胍(PBG)、乙胺嘧啶及氯喹对10株恶性疟原虫的体外作用。该10株恶性疟原虫,7株从肯尼亚儿童分离获得,3株来自东南亚(FVO为敏感株,Camp与Smith株为抗乙胺嘧啶株)。测试抗疟作用采用96井测定板,每井加25μl完全培养液(CMS)、25μl含药的CMS及200μl 1.5形(v/v)的红细胞悬液或原虫血症为0.4%的红细胞悬液。经37℃培养24小时后,每井加入25μl含0.5μCi[~3H]次黄嘌呤的CMS,继续培养18小时。然后将各井内容物移至玻璃纤维膜上,蒸馏水洗,干燥后测放射性(cpm),计算出各药抑制50%放射性掺入原虫的浓度(1D_(50))。
This article reports the in vitro effects of proguanil, cycloguanidine, p-chlorophenyl biguanide (PBG), pyrimethamine and chloroquine on 10 P. falciparums. The 10 P. falciparum isolates were obtained from seven Kenyan children, three from Southeast Asia (FVO-sensitive strains and Camp and Smith strains as anti-pyrimethamine). Tests against malaria Using a 96 well assay plate, 25 μl complete media (CMS), 25 μl drug-containing CMS and 200 μl erythrocyte suspension in 1.5 form (v / v) or 0.4% erythrocytic suspensions . After 24 hours of incubation at 37 ° C, 25 μl of CMS containing 0.5 μCi of [~ 3H] hypoxanthine was added to each well and incubation was continued for a further 18 hours. The contents of each well were then transferred to a glass fiber membrane, washed with distilled water, and radioactivity (cpm) was measured after drying. The concentration (50%) of 50% radioactively incorporated protozoa was calculated for each drug.