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体外和体内实验已证实促性腺激素释放激素(Gn-RH)和它的高效类似物有直接抑制卵巢类固醇合成的作用。卵巢细胞表面的特异受体与垂体促性腺激素细胞的受体相像,是Gn-RH作用的媒介。然而,这些仅是对啮齿类动物的观察。Gn-RH是否能直接影响人卵巢功能尚不清楚,本文报道一种Gn-RH高效类似物D-色~6-N-甲基-亮~7-去-甘~(10)-脯~9-NH-乙酰胺Gn-RH(D-Trp~6-N-methyl-L-eu~7-Des-Gly~(10)-Pro~9-NH-Ethylamide Gn-RH(WY-40,972F))抑制培养的人颗粒细胞分泌孕酮。人颗粒细胞的培养:用腹腔镜或从因病切除的卵巢的中、晚期,直径>6mm的卵泡中取颗粒细胞。每一试验是用不同对象的颗粒细胞进行。在
In vitro and in vivo experiments have demonstrated that gonadotropin-releasing hormone (Gn-RH) and its high-potency analogs have a direct effect on ovarian steroidogenesis. Specific receptors on the surface of ovarian cells and pituitary gonadotropin receptor similar, is the role of Gn-RH medium. However, these are only observations of rodents. Gn-RH can directly affect human ovarian function is unclear, this article reports a highly efficient analog Gn-RH analogue D-color ~ 6-N-methyl ~ light ~ -NH-acetamide Gn-RH (D-Trp 6-N-methyl-L-eu 7-Des-Gly 10- Pro 9- NH-Ethylamide Gn-RH (WY-40,972F)) Inhibit cultured human granulosa cells from progesterone secretion. Culture of human granulosa cells: Pelleted cells were obtained from laparoscopic or from moderate to advanced ovarian follicles> 6 mm in diameter. Each experiment is performed with different types of granulosa cells. in