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用单层平皿软琼脂克隆法证明绞股蓝总皂甙对体外培养的P388细胞克隆生长有明显的抑制作用，其IC50为（22．5±1．0）mg·L-1。为进一步探讨其抑制P388细胞生长机理，用放射性同位素掺入法观察了绞股蓝总皂成对P388细胞DNA、RNA及蛋白质合成的影响。结果表明，在绞股蓝总皂甙作用24h内，其主要抑制P88细胞DNA合成，轻度抑制RNA合成，而对蛋白质合成几乎无影响。
The single-layer petri dish soft agar cloning method was used to prove that the total saponins of Gynostemma pentaphyllum had significant inhibitory effect on the growth of clones of P388 cells in vitro. The IC50 was (22.5±1.0) mg·L-1. To further explore the mechanism of inhibiting the growth of P388 cells, the effects of Gypenosides on DNA, RNA and protein synthesis in P388 cells were observed by radioisotope incorporation. The results showed that the effect of total saponins of Gynostemma pentaphyllum against P88 cells mainly inhibited DNA synthesis and slightly inhibited RNA synthesis within 24 hours, but had little effect on protein synthesis.