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从分泌与肝细胞肝癌特异性强并具有良好人体内导向作用的鼠源性单克隆抗体的杂交瘤细胞ＨＡｂ２５中提取ＲＮＡ，逆转录成ｃＤＮＡ，用合成的寡核甘酸引物从中扩增出抗体重链可变区基因。将此基因克隆到ｐＵＣ１９质粒中，测定了此可变区基因的全序列。经计算机分析，结果表明基因长度为３６０ｂｐ，编码１２０个氨基酸，是具有功能性的抗体可变区基因，在核苷酸序列上与小鼠重链ＶＨ１８６．２家族同源性最高，属免疫球蛋白重链可变区基因９个家族中的第三族
The RNA was extracted from the hybridoma HAb25 secreting a mouse-derived monoclonal antibody with specific hepatoma specificity and good human in vivo guiding effect, reverse-transcribed into cDNA, and amplified with the synthetic oligonucleotide primer. Chain variable region genes. This gene was cloned into the pUC19 plasmid and the entire sequence of this variable region gene was determined. After computer analysis, the results showed that the gene is 360bp in length and encodes 120 amino acids. It is a functional antibody variable region gene and has the highest homology with the mouse heavy chain VH186.2 family in the nucleotide sequence. Group III of the 9 families of variable heavy-chain gene genes