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目的探讨流式细胞术检测抗肿瘤药物敏感性在临床应用的可行性,为新的抗肿瘤药物敏感性检测方法的建立,特异性抗肿瘤药物的筛查提供试验理论基础。方法应用流式细胞凋亡检测技术的SubG1法和AnnexinV法,检测10例Molt4细胞、32例临床胃肠肿瘤细胞在抗肿瘤药物作用下,不同时间点肿瘤细胞的凋亡率。并与噻唑蓝比色(MTT)法进行比较分析。结果AnnexinV法、SubG1法、MTT比色法均可检测到药物对Molt4细胞、临床胃肠肿瘤细胞杀伤率随药物作用时间延长而增加。AnnexinV法、SubG1法、MTT比色法检测药物致细胞最大杀伤率差异无统计学意义(P>0.05)。AnnexinV法检测到药物最大杀伤率的有效时间点明显早于SubG1法MTT比色法,SubG1法检测的有效时间点略早于MTT比色法。结论流式细胞术的AnnexinV法和SubG1法可作为肿瘤药物敏感性有效的检测方法。AnnexinV法比经典MTT比色法具有更敏感、快速、简洁、可靠等优点,有临床应用价值。
Objective To investigate the feasibility of using flow cytometry to detect antitumor drug sensitivity in clinical practice and to provide experimental basis for the establishment of a new antitumor drug sensitivity test and screening of specific antitumor drugs. Methods SubG1 and AnnexinV methods were used to detect the apoptosis rate of tumor cells in 10 Molt4 cells and 32 clinical gastrointestinal tumor cells under the action of antineoplastic agents at different time points. And compared with thiazole blue colorimetric (MTT) method for comparative analysis. Results AnnexinV method, SubG1 method and MTT colorimetric method could all detect that the drug killing rate of Molt4 cells and clinical gastrointestinal tumor cells increased with the prolongation of drug effect. AnnexinV method, SubG1 method, MTT colorimetric detection of drug induced cell killing rate was no significant difference (P> 0.05). AnnexinV method to detect the maximum killing rate of drugs was significantly earlier than the effective date of the SubG1 MTT colorimetric method, SubG1 method for the detection of slightly earlier than the MTT colorimetric method. Conclusion Flow cytometry AnnexinV method and SubG1 method can be used as an effective detection of drug sensitivity of oncology. AnnexinV method than the classic MTT colorimetric method is more sensitive, fast, concise, reliable, etc., has clinical value.