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目的对比研究6种 β-内酰胺类抗菌药物泰宁(TIN)、卡贝宁(CBN)、哌拉西林(PIP)、美洛西林(MZL)、头孢哌酮(CPZ)和头孢地嗪(CDZM)对2种G-杆菌大肠埃希氏菌和绿脓假单胞菌的抗生素后效应(PAE)。方法采用AVANTAGE全自动分析仪 ,应用光密度法测定PAE。结果与结论美洛西林对大肠埃希氏菌的PAE为负值或零 ,对绿脓假单胞菌的PAE很小 ,仅在4MIC时PAE达1h左右 ;哌拉西林对大肠埃希氏菌和绿脓假单胞菌的PAE均很小 ,即使在4MIC时PAE也仅有0.3h左右。在4MIC以下时 ,PAE与浓度依赖性不甚明显。头孢哌酮和头孢地嗪除了在4MIC时对大肠埃希氏菌的PAE可达1h外 ,其余PAE均很短 ,甚至没有 ,但随着抗菌药物浓度的增加 ,PAE显著增加(P<0.05) ,呈现部分浓度依赖性 ,提示在较高浓度下可望产生相对明显的PAE。一般β-内酰胺类抗菌药物对G-杆菌PAE较小 ,而泰宁和卡贝宁则较长。泰宁和卡贝宁的PAE要显著长于其它4种抗菌药物(P<0.05) ,两药对大肠埃希氏菌和绿脓假单胞菌的PAE均可达到1～3h ,同时卡贝宁的PAE要显著长于泰宁。即使在1倍MIC时 ,PAE亦显著长于其它4种药物 ,而在4倍MIC时则更长。两药对G -杆菌的PAE在1/2至4倍MIC浓度间呈显著的浓度依赖性。提示泰宁和卡贝宁可能具有与其它β-内酰胺类抗菌药物不同的PAE产生机理
OBJECTIVE To compare the effects of six kinds of β-lactam antibiotics, such as TIN, CBN, PIP, MZL, CPZ and cefodizime CDZM) on post-antibiotic effect (PAE) of two G-bacteria, Escherichia coli and Pseudomonas aeruginosa. Methods AVANTAGE automatic analyzer, the application of optical density method for the determination of PAE. RESULTS AND CONCLUSION The PAE of mezlocillin against Escherichia coli was negative or zero, and the PAE against Pseudomonas aeruginosa was very small. PAE reached about 1h at 4MIC. Piperacillin against Escherichia coli And Pseudomonas aeruginosa PAE are very small, even at 4MIC PAE only 0.3h or so. PAE is less concentration-dependent than 4MIC. Cefoperazone and cefodizime PAE were very short or even absent at 4MIC for PAE of 1h, but PAE increased significantly with the increase of antimicrobial concentration (P <0.05) , Showing a partial concentration dependence, suggesting that at higher concentrations is expected to produce relatively obvious PAE. The general β-lactam antibiotics on the G-bacteria PAE smaller, while Taining and carbenicin longer. The PAE of Taining and Carbunine was significantly longer than that of the other four antibacterials (P <0.05). The PAEs of both drugs against Escherichia coli and Pseudomonas aeruginosa were both 1-3 h, The PAE is significantly longer than Taining. Even at 1-fold MIC, PAE was significantly longer than the other 4 drugs and longer at 4-fold MIC. The PAEs of both drugs on G-bacilli were significantly concentration-dependent at 1/2 to 4 times MIC concentration. Suggesting that Taining and Carbinen may have a different mechanism of PAE production than other [beta] -lactam antibiotics