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目的探讨调控Wnt5a/Ca~(2+)/Calcineurin/NFAT通路对黑素瘤细胞增殖、侵袭能力的影响。方法体外培养慢病毒转染的黑素瘤稳转细胞系;Western blot法检测稳转细胞系中Wnt5a,NFAT,Calcineurin和VEGF蛋白的表达;CCK-8法检测稳转细胞系的增殖能力;Transwell小室实验检测稳转细胞系的侵袭能力。结果 Wnt5a蛋白的表达与Calcineurin,NFAT,VEGF蛋白的表达具有正相关性;上调Wnt5a的表达可使黑素瘤细胞增殖、侵袭能力增强,而下调Wnt5a的表达则可降低黑素瘤细胞的增殖和侵袭能力。结论调控Wnt5a/Ca~(2+)/Calcineurin/NFAT通路可影响黑素瘤细胞的增殖与侵袭,该信号通路可能参与了黑素瘤的发生、发展过程。
Objective To investigate the effects of Wnt5a / Ca ~ (2 +) / Calcineurin / NFAT pathway on the proliferation and invasion of melanoma cells. METHODS: The lentiviral-transfected melanoma cells were cultured in vitro. The expression of Wnt5a, NFAT, Calcineurin and VEGF in stable cell lines was detected by Western blot. The proliferation of stable cell lines was detected by CCK-8 assay. Transwell Chamber assay detects metastatic cell line invasion. Results The expression of Wnt5a protein was positively correlated with the expression of Calcineurin, NFAT and VEGF protein. The up-regulation of Wnt5a expression could increase the proliferation and invasion ability of melanoma cells. However, the down-regulation of Wnt5a expression could reduce the proliferation and proliferation of melanoma cells Invasive ability. Conclusion The regulation of Wnt5a / Ca ~ (2 +) / Calcineurin / NFAT pathway may affect the proliferation and invasion of melanoma cells, which may be involved in the pathogenesis and progression of melanoma.