为满足小麦大规模分子标记检测的需要,以小麦幼叶为材料,探索在无液氮条件下,不用研磨,直接用沸水水浴提取小麦总DNA的新方法。结果表明,该方法与常规提取法相比,提取的总DNA经分光光度计及琼脂糖凝胶电泳检测发现质量较好,用其为模板进行SSR-PCR扩增,条带清晰,获得较好的结果。证明这种快速提取小麦DNA的方法省时、省力,适合大量的小麦分子检测需要。 In order to meet the needs of large-scale molecular marker detection of wheat, young wheat leaves were used as materials to explore a new method for extracting total DNA from wheat without using liquid nitrogen and directly grinding with boiling water. The results showed that compared with the conventional extraction method, the total DNA extracted by the method was better detected by spectrophotometer and agarose gel electrophoresis, and was used as a template for SSR-PCR amplification with clear bands and better result. This method of rapid DNA extraction from wheat proved to be time-saving and labor-saving, suitable for a large number of wheat molecular detection needs.