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背景:目前经皮椎体成形技术已广泛应用于治疗老年骨质疏松椎体压缩性骨折以及椎体肿瘤并取得良好疗效,但仍有一些问题需关注和探讨。目的:观察聚甲基丙烯酸甲酯骨水泥对脊柱转移瘤细胞周期变化和细胞凋亡的影响,分析其对脊柱转移瘤的体外抗肿瘤效应。方法:原代培养脊柱转移瘤细胞,将其分为对照组、拉丝期聚甲基丙烯酸甲酯骨水泥组、冷却后聚甲基丙烯酸甲酯骨水泥组,药物处理24h后利用流式细胞术观察凋亡率以及细胞周期,通过SYBRGreenⅠ荧光实时定量-聚合酶链反应法检测肿瘤细胞Bax和Caspase-3mRNA表达的变化。结果与结论:聚甲基丙烯酸甲酯骨水泥有促进脊柱转移瘤细胞凋亡的作用,对照组、拉丝期聚甲基丙烯酸甲酯骨水泥组、冷却后聚甲基丙烯酸甲酯骨水泥组的凋亡率分别为(3.80±1.09)%,(43.46±8.55)%,(28.77±9.39)%,两骨水泥组均能明显提高细胞凋亡率,拉丝期聚甲基丙烯酸甲酯骨水泥组作用更明显(P<0.05)。两骨水泥组均能明显减少G0/G1期细胞,增加S期细胞(P<0.05)。拉丝期聚甲基丙烯酸甲酯骨水泥组作用于脊柱转移瘤细胞后Bax、Caspase-3mRNA的表达显著高于对照组(P<0.05)。提示聚甲基丙烯酸甲酯骨水泥可在体外细胞水平诱导肿瘤细胞凋亡,拉丝期聚甲基丙烯酸甲酯骨水泥作用更加显著。
BACKGROUND: Percutaneous vertebroplasty has been widely used in the treatment of elderly patients with osteoporotic vertebral compression fractures and vertebral tumors and achieved good results. However, there are still some problems that need attention and discussion. Objective: To observe the effect of polymethylmethacrylate (PMMA) cement on the cell cycle and cell apoptosis of spinal metastases, and to analyze its anti-tumor effect in vitro. Methods: Primary cultured spine metastasis cells were divided into control group, drawing period polymethylmethacrylate cement group, cooling polymethylmethacrylate cement group, drug treatment 24h after flow cytometry The apoptosis rate and cell cycle were observed. The changes of Bax and Caspase-3 mRNA expression in tumor cells were detected by real-time quantitative PCR with SYBR Green I fluorescence. RESULTS AND CONCLUSION: Polymethylmethacrylate (BMMA) cement could promote the apoptosis of spinal metastasis cells. In the control group, the polymethylmethacrylate cement for drawing period, the polymethylmethacrylate cement for cooling (3.80 ± 1.09)%, (43.46 ± 8.55)% and (28.77 ± 9.39)%, respectively. Both of the two groups of cement could significantly increase the rate of apoptosis, while the percentage of apoptosis in the polymethylmethacrylate cement group The effect is more obvious (P <0.05). Bone cement group can significantly reduce the G0 / G1 phase cells, increase S phase cells (P <0.05). The expression of Bax and Caspase-3mRNA in the spine metastasis cells was significantly higher than that in the control group (P <0.05) during the drawing period of polymethylmethacrylate cement group. Tip polymethylmethacrylate cement can induce tumor cell apoptosis at the cellular level in vitro, and the effect of polymethylmethacrylate cement during drawing is more significant.