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作者应用染色配体层析法从百日咳杆菌的培养上清中提取百日咳毒素(PT)和丝状血凝素(FHA),并研究了用水溶性1-乙基-3-(3-二甲氨丙基)-碳化二亚胺(EDAC)进行类毒化的条件。应用SS培养基加环糊精和环糊精液体(CL)培养基制备PT和FHA。用Blue Sepharose层析从培养上清中提取抗原。SS培养基加环糊精产生的PT是FHA的2.5倍,
The authors extracted pertussis toxin (PT) and filamentous hemagglutinin (FHA) from the culture supernatant of Bordetella pertussis by using the dye-ligand chromatography. The effects of water-soluble 1-ethyl-3- Propyl) -carbodiimide (EDAC). PT and FHA were prepared using SS medium plus cyclodextrin liquid (CL) medium. Antigen was extracted from the culture supernatant by Blue Sepharose chromatography. The PT produced by SS medium plus cyclodextrin was 2.5 times that of FHA,