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利用快速蛋白质液相色谱技术 (简称 FPL C技术 )对 3个典型黄萎病菌系 T9、VD8和 SS-4的外泌毒蛋白进行层析分离 ,皆得到两个致萎蛋白峰 :峰 致萎力弱 ,棉苗受害症状属黄斑型 ;峰 致萎力极强 ,症状属青枯型。 3个菌系强致萎峰 毒蛋白的百分含量由高到低依次为 T9、VD8、SS-4。对各菌系致萎峰蛋白做氨基酸组分测定 ,结果显示 :13个菌系的致萎峰 毒蛋白中的碱性组氨酸百分含量很高 ,平均达 14 .76% ,远大于峰 毒蛋白中的组氨酸含量 ,其平均值仅 3 .74 % ;2 3个菌系各致萎峰皆测不出甲硫氨酸 ,酪氨酸仅在 T9的峰 中含 0 .13 % ,其余各峰含量均为零 ,各峰的半胱氨酸含量很低 ,都在 1%以下。还利用高效液相色谱技术估测致萎峰蛋白的分子量 ,并探讨了黄萎病菌致萎毒蛋白的有关结构功能特征。
Chromatographic separation of the exocrine proteins of three typical Verticillium dahliae strains T9, VD8 and SS-4 using fast protein liquid chromatography (FPL C) revealed two peaks of withering protein peaks Weak, the symptoms of cotton seedlings suffer from macular type; peak caused by extremely strong wilt symptoms are scape. The results showed that the percentages of three strains of phytopathogenic fodder peaked from high to low were T9, VD8 and SS-4. The results showed that the percentages of basic histidine in the fusogenic peak proteins of 13 strains were high, averaging 14.76%, much larger than the peak The average content of histidine in toxic protein was only 3.74%. Methionine was not detected in all 23 strains, but tyrosine contained only 0.13% in the peak of T9. , The rest of the peak content is zero, the peak cysteine content is very low, are less than 1%. The molecular weight of peaked protein was also estimated by HPLC, and the structural and functional characteristics of verticillin-wilt protein were also discussed.