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目的采用细胞凋亡、增殖细胞核抗原的检测方法，探讨其在宫颈鳞癌、腺癌中的表达及其诊断价值。方法用细胞凋亡ＤＮＡ片断原位末端检测组织切片标记法及增殖细胞核抗原免疫组化染色法，检测２８例宫颈癌的档存蜡块切片，并与５例正常宫颈组织蜡块切片作对照统计。结果宫颈鳞癌及腺癌的两种方法检测结果与正常宫颈比较统计分析结果，差别都有显著意义（Ｐ＜００１），宫颈鳞癌与腺癌之间两种方法检测结果统计处理差别无显著意义（Ｐ＞００５）。结论细胞凋亡、增殖细胞核抗原检测方法在不同类型的宫颈癌病例中都能表达。该两种方法将有助于判断肿瘤细胞生长的速度，为临床化疗药物的发展开拓一新前景
Objective To investigate the expression and diagnostic value of apoptosis and proliferating cell nuclear antigen in cervical squamous cell carcinoma and adenocarcinoma. Methods Apoptotic DNA fragment was used to detect the tissue sections in situ and proliferating cell nuclear antigen immunohistochemical staining. A total of 28 cases of cervical cancer were examined and the paraffin-embedded sections were compared with 5 cases of normal cervical tissue. . Results The results of the two methods of cervical squamous cell carcinoma and adenocarcinoma were statistically different from those of normal cervix (P<001). There were statistical differences in the results of the two methods between cervical squamous cell carcinoma and adenocarcinoma. No significant significance (P>005). Conclusions Apoptosis and proliferating cell nuclear antigen detection methods can be expressed in different types of cervical cancer cases. The two methods will help determine the speed of tumor cell growth and open up new prospects for the development of clinical chemotherapy drugs.