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目的探讨蓝莓花色苷单体(矢车菊-3-O-半乳糖苷)对老龄小鼠学习记忆功能的改善作用。方法15月龄昆明小鼠24只,按体重随机分为对照(control,Cont)、蓝莓提取物(blueberry extracts,BBE)和蓝莓花色苷单体(blueberry monomer,BBM)3组。对照组小鼠灌胃蒸馏水;蓝莓提取物及其单体的灌胃剂量分别为200和50mg/kgbw·d,干预期6w。干预结束后,用Morris水迷宫实验和避暗实验检测小鼠的学习记忆能力;同时检测脑组织脂褐质含量以及血清、脑组织中丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性的变化。结果与对照组比较,BBE组和BBM组小鼠的避暗潜伏期明显延长(P<0.05);Morris水迷宫中的逃避潜伏期明显缩短(P<0.05),在平台象限的持续时间明显延长(P<0.05),穿越次数增加(P<0.05);同时脑组织中脂褐质含量明显降低(P<0.01);血清、脑组织中MDA含量显著下降(P<0.05,P<0.01),而SOD活性明显升高(P<0.01)。上述变化在BBE组和BBM组之间无显著性差异(P>0.05)。结论蓝莓矢车菊-3-O-半乳糖苷单体能够显著改善老龄小鼠学习记忆功能,其作用机制与减少脑组织中脂褐质的积累、提高抗氧化防御系统功能有关。
Objective To investigate the effect of anthocyanin monomer (blueberry-3-O-galactoside) on learning and memory in aging mice. Methods Twenty-four Kunming mice, 15 months old, were randomly divided into control (cont), blueberry extracts (BBE) and blueberry monomer (BBM) groups. The mice in the control group were fed with distilled water; the intragastric doses of blueberry extract and its monomer were 200 and 50 mg / kg bw · d, respectively, and the intervention period was 6 weeks. After the intervention, the learning and memory abilities of mice were detected by Morris water maze test and dark-avoidance test. The levels of lipofuscin, serum malondialdehyde (MDA) and superoxide dismutase (SOD) ) Activity changes. Results Compared with the control group, the latency to avoid darkness was significantly longer in the BBE and BBM groups (P <0.05), and the escape latency in the Morris water maze was significantly shorter (P <0.05) and prolonged in the platform quadrant (P (P <0.05), and the number of traversing times increased (P <0.05). At the same time, the content of lipofuscin in brain tissue decreased significantly (P <0.01), and the content of MDA in serum and brain tissue decreased significantly Activity was significantly increased (P <0.01). The above changes in BBE group and BBM group no significant difference (P> 0.05). Conclusion Blueberry cornflower -3-O-galactoside monomer can significantly improve learning and memory in aging mice, and its mechanism of action is related to reducing the accumulation of lipofuscin in brain tissue and enhancing the function of antioxidant defense system.