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目的探讨重复性饥饿/再投喂大鼠摄取高脂食物后,瘦素(leptin)与核酸结合蛋白2(NUCB 2)的表达变化。方法重复性饥饿/再投喂大鼠6周后,改为每天喂以高脂饲料(饥饿高脂组),实验设高脂对照组和基础对照组,喂养至12周。比较各组大鼠Lee′s指数和脂体比,测定血脂和血清leptin含量,检测下丘脑NUCB 2表达水平。结果饥饿组血清leptin水平为(3.18±0.54)ng/mL,明显高于对照组(2.44±0.45)ng/mL(P<0.05);NUCB 2阳性表达亦明显高于对照组(P<0.01);饥饿高脂组Lee′s指数、脂体比、血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL)及leptin水平均明显低于高脂对照组而高于基础对照组(P<0.05)。结论重复性饥饿/再投喂处理可降低高脂饮食诱导的肥胖程度,减轻leptin抵抗,但会引起肥胖大鼠下丘脑NUCB 2表达减弱。
Objective To investigate the expression of leptin and nucleic acid binding protein 2 (NUCB 2) in rats fed with high-fat diet after repeated starvation / re-feeding. Methods After repeated starvation / re-feeding for 6 weeks, the rats were fed with high-fat diet (starving hyperlipidemia group) every day. The experimental group was given high-fat control group and basic control group and fed for 12 weeks. The Lee’s index and lipid ratio of rats in each group were compared, and the levels of serum lipids and serum leptin were measured to detect the expression of NUCB 2 in the hypothalamus. Results The level of leptin in the starvation group was significantly higher than that in the control group (3.18 ± 0.54) ng / mL (2.44 ± 0.45 ng / mL, P <0.05). The positive expression of NUCB 2 in the starvation group was also significantly higher than that in the control group ; Lee’s index, body fat ratio, serum total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL) and leptin levels in starvation hyperlipidemia group were significantly lower than those in hyperlipidemic control group Control group (P <0.05). Conclusions Repeated starvation / re-feeding can reduce the obesity induced by high-fat diet and reduce leptin resistance, but it can reduce the expression of NUCB 2 in hypothalamus of obese rats.