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目的 :建立豚鼠耳蜗血管纹 (SV)组织块缘细胞 (MCs)的培养方法 ,为进一步研究药物耳毒性及其作用机制奠定基础。方法 :2 6只豚鼠按SV培养时间随机分成 4组 :2 4h组 (n =8) ;72h组 (n =8) ;>72h组 (n =8) ;对照组 (新鲜SV固定组 ,n =2 )。显微解剖数段连同螺旋韧带的SV组织块 ,置于 5 %CO2 / 95 %空气的二氧化碳恒温 (37℃ )培养箱中进行培养 ,分别进行形态学和组织学观察。结果 :培养 2 4hSV组织块保持良好活性 ,其组织学结构与新鲜固定的SV结构无明显差异 ;培养 72hSV组织块与新鲜固定的SV在组织学结构方面有显著性差异 ,不能观察到正常的SV结构 ,组织结构松散 ,缘细胞从组织块离心性生长出来 ;从SV组织块培养出的缘细胞能在培养皿内存活 13d。结论 :采用组织块培养技术 ,成功地建立了豚鼠耳蜗SV组织块的缘细胞培养方法 ;培养 2 4h的SV组织块光镜下保持了良好活性和正常组织学结构 ,可用来进一步研究药物耳毒性及其作用机制。
OBJECTIVE: To establish a culture method of guinea pig stromal cells (MCs) in the stria vascularis (SV) tissue, which will lay the foundation for further study on drug ototoxicity and its mechanism of action. Methods: Twenty-six guinea pigs were randomly divided into 4 groups according to SV culture time: 24 h (n = 8), 72 h (n = 8), and> 72 h = 2). The microdissected sections along with the SV ligament of the spiral ligament were cultured in a constant temperature (37 ° C) carbon dioxide incubator at 5% CO2 / 95% air and morphologically and histologically. RESULTS: Twenty-four hours after incubation, the histological structure of SVS showed no significant difference with that of fresh fixed SV. There was a significant difference in the histological structure between the cultured 72 h SV tissue and the fresh fixed SV, and the normal SV The structure and tissue structure were loose, and the marginal cells grew out of the tissue blocks centrifugation. The marginal cells cultured from the SV tissue block could survive for 13 days in the culture dish. CONCLUSION: The method of tissue culture was used to establish the method of culturing the cochlear SV tissue of guinea pigs. The culture of 24 h SV was performed under light microscope to maintain good activity and normal histological structure, which could be used to further study the ototoxicity And its mechanism of action.