论文部分内容阅读
对培养7 d的绿粘帚霉(Gliocladium.virens)F051分生孢子悬浮液用15 W紫外灯,距离为30 cm处进行照射,并结合含药培养基培养,筛选得到了2株绿粘帚霉(G.virens)的多菌灵耐药性菌株。试验结果表明:紫外照射3min时,绿粘帚霉F051分生孢子的致死率达89.3%;当照射时间达到5 min时,孢子的存活率为0,故选择照射3min为本试验的最适诱变剂量;使F051致死的多菌灵最低终浓度为2μg/mL;诱变菌株与亲本菌株相比,抗药性提高了4倍,产孢能力更强,菌丝生长更为密集,这有利于生防真菌更快的抑制植物病原真菌的生长,减少农药的使用量,缓解化学防治对环境的压力。
On the 7th day, the suspension of Gliocladium.virens F051 conidia was irradiated with 15 W UV lamp at a distance of 30 cm, and cultured with drug-containing medium. Two strains of green viscose Carbendazim-resistant strains of G. virens. The results showed that the lethality of conidia of Gliocladium sp. F051 was 89.3% when exposed to ultraviolet light for 3 min. When the irradiation time reached 5 min, the survival rate of spores was 0, so the optimal irradiation was selected for 3 min The lowest dose of carbendazim at F051 lethality was 2μg / mL. The mutagenic strains showed a 4-fold increase in drug resistance and a stronger sporulation ability than the parent strain, resulting in a more dense mycelium growth, which is in favor of Biocontrol fungi faster inhibit the growth of plant pathogenic fungi, reducing the amount of pesticides used to ease the pressure of chemical control on the environment.