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研究根据草莓基因组和月季基因组的同线性关系,高通量开发月季SSR共显性标记。利用MISA软件对总长206.8Mb的草莓基因组序列进行扫描,共识别49 029个SSR位点,平均每4.2 kb包含1个SSR位点。利用primer3软件成功对21 288个SSR位点设计引物。利用中国古老月季品种‘月月粉’(Rosa chinensis‘Old blush’)和园艺品种‘无刺光叶蔷薇’(Rosa wichuriana‘Basye’s thornless’),随机选取300对SSR引物进行PCR扩增,40对引物获得清晰条带,扩增率为13.3%,发现其中8个可转移的SSR位点位于草莓基因组第六染色体63 959 bp的基因组区间。研究结果为月季和草莓间的比较基因组学研究提供了分子证据,为月季生物学研究提供宝贵的标记资源。
Based on the same linear relationship between strawberry genome and rose genome, high-throughput development of a seasonal co-dominant marker of SSR was studied. MISA software was used to scan the 206.8Mb genome sequence of the strawberry genome. A total of 49,029 SSR loci were identified, with an average of one SSR locus per 4.2 kb. Primer was successfully designed for 21 288 SSR loci using primer3 software. Three hundred and thirty SSR primers were randomly selected for PCR amplification using Rosa chinensis’Old blush ’and the horticultural variety’ Rosa wichuriana’Basye’s thornless’ The primers were cleaved with 13.3% amplification. Eight of the 8 SSR loci were found in the genome of 63 959 bp on the sixth chromosome of strawberry genome. The results provide molecular evidence for comparative genomics studies between rose and strawberry and provide valuable marker resources for the study of rose biology.