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目的：探讨无能Ｔ细胞与凋亡的关系。方法：利用Ｂ７１单抗和环孢素Ａ（ＣｓＡ）联用处理ＡＰＣ即Ｔ细胞系统，在体外诱致抗原特异性Ｔ细胞无能，用ＰＩ染色法经ＦＡＣＳ分析了无能Ｔ细胞的凋亡，用ＲＴＰＣＲ法检测了凋亡细胞ＦａｓｍＲＮＡ的表达水平。结果：无能Ｔ细胞在１、３、５、１０ｄ细胞凋亡的百分率依次为２．１６％、１１．２８％、１９．２７％、４１．２２％。当加入１００Ｕ／ｍｌＩＬ２维持培养，无能Ｔ细胞未显示明显凋亡。无能Ｔ细胞活化后经ＰＨＡ、ＰＭＡ＋Ａ２３１８７、ＣＤ３单抗再次诱导，２４ｈ便发生明显凋亡，凋亡百分率分别为３４．６４％、２１．２７％、１５．０７％，活化后的Ｔ细胞及无能Ｔ细胞凋亡时ＦａｓｍＲＮＡ上调表达。结论：无能Ｔ细胞在体外是逐渐发生凋亡的，Ｆａｓ／ＦａｓＬ参与细胞凋亡过程
Objective: To investigate the relationship between incompetent T cells and apoptosis. Methods: The combination of B71 monoclonal antibody and cyclosporine A (CsA) treatment of APC or T cell system in vitro induced antigen-specific T cell incompetence, PI staining by FACS analysis of incompetent T cell apoptosis, The expression of Fas mRNA in apoptotic cells was detected by RT-PCR. Results: The percentages of apoptosis of incompetent T cells at 1, 3, 5 and 10 days were 2.16%, 11.28%, 19.27% and 41.22%, respectively. When adding 100U / ml IL 2 maintenance culture, incapable T cells showed no significant apoptosis. After activation of incompetent T cells, the cells were induced again by PHA, PMA + A23187 and CD3 monoclonal antibody, and obvious apoptosis occurred at 24h, with the percentages of apoptosis being 34.64%, 21.27% and 15.07%, respectively. The activated T cells and impotence T-cell apoptosis FasmRNA up-regulated expression. CONCLUSION: Incomplete T cells are gradually apoptotic in vitro, and Fas / FasL is involved in the process of apoptosis