Objective:Evaluate the application values of 99mTc-2 β[N, N′-bis(2-mercaptoethy1)ethylenediamino]methyl, 3β-(4-chlorophenyl)tropane(TRODAT-1) dopamine transporter (DAT) SPECT imaging in children autism, and offer the academic foundation to etiology, mechanism and clinical therapy of autism. Methods:Ten autistic children and ten healthy controls were examined with 99mTc-TRODAT-1 DAT SPECT imaging. Striatal specific uptake of 99mTc-TRODAT-1 was calculated with region of interest analysis according to the ratios between striatum and cerebellum[(STR-BKG)/BKG]. Results:There was no difference in semiquantitative dopamine transporter between bilateral striatum in autistic children (P=0.562) and in normal controls (P=0.573); dopamine transporter in brain of patients with autism increased more significantly than that in normal controls (P=0.017).Conclusion:Dopaminergic nervous system is dysfunction in human brain with children autism, and DAT 99mTc-TRODAT-1 SPECT imaging on human brain will help the imaging diagnosis of children autism. Objective: Evaluate the application values ​​of 99mTc-2 β [N, N’-bis (2-mercaptoethy1) ethylenediamino] methyl, 3β- (4-chlorophenyl) tropane , and offer the academic foundation to etiology, mechanism and clinical therapy of autism. Methods: Ten autistic children and ten healthy controls were examined with 99mTc-TRODAT-1 DAT SPECT imaging. Striatal specific uptake of 99mTc-TRODAT-1 was calculated with region of interest analysis according to the ratios between striatum and cerebellum [(STR-BKG) / BKG]. Results: There was no difference in semiquantitative dopamine transporter between bilateral striatum in autistic children (P = 0.562) and in normal controls ); dopamine transporter in brain of patients with autism increased more significantly than that in normal controls (P = 0.017) .Conlusion: Dopaminergic nervous system is dysfunction in human brain with children autism, and DAT 99mTc-TRODAT-1 SPECT imaging on human brain wil l help the imaging diagnosis of children autism.