【摘 要】
：
Ascorbate peroxidase (APX) plays a key role in scavenging reactive oxygen species (ROS) in higher plants. However, there is very little information available on the APXs in kiwifruit (Actinidia), which is an economically and nutritionally important hortic
【机 构】
：
Key Laboratory for Bio-resource and Eco-environment of Ministry of Education,College of Life Science
论文部分内容阅读
Ascorbate peroxidase (APX) plays a key role in scavenging reactive oxygen species (ROS) in higher plants. However, there is very little information available on the APXs in kiwifruit (Actinidia), which is an economically and nutritionally important horticultural crop with exceptionally high ascorbic acid (AsA) accumulation. This study aims to identify and characterize two cytosolic APX genes (AcAPX1 and AcAPX2) derived from A. chinensis \'Hongyang\'. The constitutive expression pattern was determined for both AcAPX1 and AcAPX2, and showed relatively higher expression abundances of AcAPX1 in leaf and AcAPX2 in root. Transcript levels of AcAPX1 and AcAPX2 were increased in kiwifruit roots treated with NaCl. Subcellular localization assays using GFP-fusion proteins in Arabidopsis protoplasts showed that both AcAPX1 and AcAPX2 are targeted to the cytosol. Recombinant AcAPX1 or AcAPX2 proteins were successfully expressed in the prokaryotic expression system and their individual ascorbate peroxidase activities were determined. Finally, constitutive over-expression of AcAPX1 or AcAPX2 could dramatically increase total AsA, glutathione level and salinity tolerance under NaCl stress in Arabidopsis thaliana. Our findings revealed that cytosolic AcAPX1/2 may play an important protective role in the responses to unfavorable environmental stimuli in kiwifruit.
其他文献
为研究牛的抗病能力,探究荷斯坦牛Nramp1基因所编码蛋白的结构与功能,本研究采用生物信息学分析的方法对荷斯坦牛Nramp1基因编码蛋白质的理化性质、疏水性/亲水性、信号肽跨膜区、跨膜结构域、N-糖基化位点和磷酸化位点、蛋白质二级结构、三级结构及蛋白质互作进行分析和预测.结果表明,荷斯坦牛Nramp1基因所编码蛋白全长548个氨基酸,等电点为6.39,是一种不稳定的疏水蛋白.二级结构以α螺旋和无规则卷曲为主,具有41个潜在的磷酸化位点和1个N-糖基化位点,且存在与之相关的蛋白.本研究结果对于深入研究荷斯坦
Although the complete nucleotide sequence of strawberry vein banding virus (SVBV) has been determined and bioinformatic analysis has revealed that the SVBV genome could encode seven proteins, the precise function of each protein is unclear. This study pro
本文基于美国、加拿大、欧盟、澳大利亚、新西兰等世界主要奶业发达国家或地区的数据,对全球奶牛胚胎、冻精等遗传物质的进出口情况进行了分析,并结合主要进口来源国的出口数据,对我国奶牛遗传物质的进口情况进行了简述,供行业参考.
Whole genome duplication (WGD) and tandem duplication (TD) are important modes of gene amplification and functional innovation, and they are common in plant genome evolution. We analyzed the genomes of three Solanaceae species (Solanum lycopersicum, Capsi
后备奶牛健康生长对其未来生产性能具有重要影响.调研结果表明,呼吸系统疾病和营养代谢疾病是导致后备奶牛发病的主要原因,占比分别为46.0％和38.4％.而后备奶牛中发病最多的为哺乳犊牛,占比达51.4％.目前,哺乳犊牛发病率正在逐年降低,从2015年的43.3％降至2019年的25.4％.此外调研发现,造成后备奶牛被动淘汰的主要原因是呼吸系统疾病、营养代谢疾病以及繁殖疾病,占比共为79.1％,并且被动淘汰主要发生在断奶犊牛和青年牛阶段,分别占后备奶牛被动淘汰总数的36.0％和23.8％.调研结果在一定程度上
本研究检索并分析了全自动挤奶机器人领域专利的申请地域,结果表明,全自动挤奶机器人技术掌握在少数几个国家手中,瑞典、荷兰、美国、德国在全自动挤奶机器人领域处于领先及核心地位,这四个国家的主要专利权人分别是利拉伐、LELY、Technologies Holdings Corp、GEA.欧洲和美国是目前全自动挤奶机器人技术最活跃、最受重视的目标市场.荷兰是全自动挤奶机器人技术最大输出国,其次是瑞典.中国的技术主要在本国申请专利,海外布局极少.
MicroRNA (miRNA) has vital regulatory effects on the proliferation, differentiation and secretion of ovarian granulosa cells, but the role of miR-99a-5p in goat ovarian granulosa cells (GCs) is unclear. Both miR-99a-5p and Frizzled-5 (FZD5) were found to
Pakchoi (Brassica campestris L. ssp. chinensis) is an important leafy vegetable. Various light spectra, especially red and blue light, play vital roles in the regulation of nitrate metabolism. Information on the effects of red and blue light on nitrate me
为研究LPCV牛舍夏季舍内环境变化规律,为LPCV牛舍提供合理的湿帘风机运行方案,本文以沈阳地区某六排低屋面横向通风牛舍为研究对象,采用计算流体动力学(Computational fluid dynamics,CFD)方法,分别在沈阳夏季设计工况和极端最热工况下对舍内环境进行三维数值模拟,分析两种工况条件下舍内的温度和速度分布规律,确定两种工况下的湿帘风机运行方案.模拟结果表明,沈阳地区夏季设计工况条件下,风机全开,湿帘喷淋水采用常温水20℃时,舍内环境可满足舍内奶牛舒适性要求;在极端最热工况条件下,风机
The root system architecture (RSA) of a crop has a profound effect on the uptake of nutrients and consequently the potential yield. However, little is known about the genetic basis of RSA and resource adaptive responses in wheat (Triticum aestivum L.). He