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目的获得白花丹参丙酮酸脱羧酶(SmPDC)全长基因,分析该基因在白花丹参不同组织部位,以及缺氧胁迫处理后的该基因表达差异。方法利用cDNA文库筛选获得SmPDC基因全长,利用半定量RT-PCR,分析SmPDC基因在白花丹参不同部位的表达情况,及缺氧处理条件下的表达情况。结果获得的SmPDC基因由2 190个核苷酸组成,编码605个氨基酸,蛋白相对分子质量约6.485×104,等电点pI 5.49;半定量RT-PCR检测,该基因在丹参的根中表达量最高,其次是茎和叶;缺氧胁迫处理会诱导该基因的表达,随胁迫时间延长表达量逐渐增加。结论白花丹参SmPDC基因是PDC家族新成员,其功能与植物耐缺氧代谢途径有关。
OBJECTIVE: To obtain the full length gene of pyruvate decarboxylase (SmPDC) from Salvia miltiorrhiza Bge.The objective of this study was to analyze the difference of expression of this gene in different tissues of Salvia miltiorrhiza Bunge and the treatment with hypoxia stress. Methods The full-length SmPDC gene was screened by cDNA library. The expression of SmPDC gene in different parts of Salvia miltiorrhiza Bunge was analyzed by semi-quantitative RT-PCR, and the expression of SmPDC gene under hypoxia condition was analyzed. Results The SmPDC gene was composed of 2 190 nucleotides and encoded a protein of 605 amino acids with a molecular weight of 6.485 × 104 and an isoelectric point of pI of 5.49. Semi-quantitative RT-PCR showed that the SmPDC gene expressed in root of Salvia miltiorrhiza Followed by stems and leaves. The expression of this gene was induced by hypoxia stress, and the expression gradually increased with prolonging of stress time. Conclusion The Salvia miltiorrhiza SmPDC gene is a new member of PDC family, and its function is related to plant hypoxia-tolerance pathway.