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为研究胰腺癌的转移机理，在我校原建立的人胰腺癌ＪＦ３０５ 细胞系基础上，利用细胞克隆，细胞电泳，流式细胞仪及人癌裸小鼠原位移植技术，建立了具有不同转移潜能的人胰腺癌ＪＦ３０５ 单克隆细胞亚系和具有不同转移潜能的人胰腺癌单克隆细胞亚系裸小鼠原位移植模型。结果表明该单克隆细胞亚系及其肿瘤移植模型的建立，为探讨胰腺癌转移机理提供了较理想的研究方法。
In order to study the mechanism of pancreatic cancer metastasis, based on the original human pancreatic cancer JF305 cell line established in our school, the use of cell cloning, cell electrophoresis, flow cytometry and human cancer nude mice orthotopic transplantation technology, established a different transfer Potential human pancreatic cancer JF305 monoclonal cell subline and human pancreatic cancer monoclonal cell sublineage nude mice orthotopic transplantation model with different metastatic potential. The results show that the establishment of this monoclonal cell subline and its tumor transplantation model provides an ideal method for exploring the mechanism of pancreatic cancer metastasis.