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目的：研究牛膝多糖对巨噬细胞的激活作用。方法：０３１２ 、０６２５ 、１２５０ 、２５００ 和５０００ ｍｇｍｌ 牛膝多糖对体外培养的人胸腔巨噬细胞进行诱导培养２４ ｈ ，用全自动生化分析仪测定胸腔巨噬细胞内乳酸脱氢酶与酸性磷酸酶活性，用放射免疫分析法测定胸腔巨噬细胞内肿瘤坏死因子α与白细胞介素６ 的含量。结果：牛膝多糖能上调胸腔巨噬细胞内乳酸脱氢酶和酸性磷酸酶活性，并能显著诱导胸腔巨噬细胞表达肿瘤坏死因子α和白细胞介素６ ，而且对肿瘤坏死因子α的诱导表达具有显著的剂量效应。结论：牛膝多糖可诱导巨噬细胞表达细胞因子，并对巨噬细胞具有激活作用。
Objective: To study the activation of macrophage by Achyranthes bidentata polysaccharide. Methods: Human thoracic macrophages cultured in vitro were cultured for 24 h at 0312, 0625, 1250, 2500 and 5000 mg Achyranthes bidentata polysaccharides. The thorax was measured by automatic biochemical analyzer Lactate dehydrogenase and acid phosphatase activity in macrophages were determined by radioimmunoassay. The levels of tumor necrosis factor-α and interleukin-6 in pleural macrophages were determined by radioimmunoassay. Results: Achyranthes bidentata polysaccharide can increase the lactate dehydrogenase and acid phosphatase activity in the pleural macrophages, and can significantly induce thoracic macrophages expression of tumor necrosis factor α and interleukin 6, and the tumor necrosis factor α Induced expression of a significant dose effect. Conclusion: Achyranthes bidentata polysaccharides can induce macrophages to express cytokines and activate macrophages.