采用半补齐方法建立棉铃虫多核衣壳型多角体病毒基因组文库,通过对插入片段进行克隆鉴定和序列分析,获得了38k基因。该基因上游具有晚期调控保守序列TTAAG,是一个晚期表达基因,基因阅读框为903bp,共编码300个氨基酸,氨基酸序列同源性分析结果表明其与α类杆状病毒的同源性较高,有较近的亲缘关系。氨基酸高级结构的分析表明其与与磷酸酶结构相似性达到95%,与病毒核衣壳的组装有关。 The genome of Helicoverpa armigera multi-nucleocapsid polyhedrosis virus was constructed by semi-complementation method. The 38k gene was obtained by clone identification and sequence analysis of the inserted fragment. The gene has a late regulatory TTAAG, which is a late-stage expression gene with a 903bp open reading frame and encodes a protein of 300 amino acids in length. The amino acid sequence homology analysis indicated that TTAAG has high homology with α-type baculovirus, Have a closer relationship. Analysis of the amino acid structure shows that it has 95% similarity with phosphatase structure, which is related to the assembly of the virus nucleocapsid.