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目的检测血管内皮生长因子受体-3(vascular endothelial growth factor receptor-3,VEGFR-3)在非小细胞肺癌癌组织及淋巴结组织中的表达水平,探讨VEGFR-3与非小细胞肺癌淋巴结转移的相关性。方法收集非小细胞肺癌患者术后肺组织标本52例,淋巴结196枚;良性肺疾病患者肺组织标本10例,淋巴结8枚。分别应用半定量反转录聚合酶链反应(RT-PCR)及免疫组化法检测肺组织和淋巴结组织中的VEGFR-3 mRNA及蛋白的表达量,同时测定微淋巴管密度。结果 VEGFR-3 mRNA在淋巴结转移阳性组的淋巴结组织中的表达水平低于淋巴结转移阴性组的淋巴结(P<0.05),而在淋巴结转移阳性组的肺癌组织中的表达水平与淋巴结转移阴性组肺癌组织比较无显著区别(P>0.05)。在淋巴结转移阳性组中,VEGFR-3 mRNA在阳性淋巴结与阴性淋巴结中的表达水平无显著区别(P>0.05)。VEGFR-3不仅在淋巴管内皮细胞表达,在小血管内皮及肿瘤细胞胞浆也有表达。VEGFR-3阳性管计数在癌周组织显著高于癌组织,在淋巴结转移组显著高于淋巴结未转移组。VEGFR-3蛋白表达阳性组与阴性组比较,微淋巴管密度及淋巴结转移都显著升高。结论 VEGFR-3 mRNA及蛋白的表达水平与肺癌淋巴结转移有显著相关性,在预测肺癌的淋巴结转移、完善分子分期、指导临床治疗上有重要意义。微淋巴管密度是肿瘤淋巴道转移的关键因素,其升高预示着淋巴管生成和淋巴结转移的发生,可作为肿瘤监测和预后的指示因子。
Objective To detect the expression of vascular endothelial growth factor receptor-3 (VEGFR-3) in non-small cell lung cancer and lymph node tissue, and to investigate the relationship between VEGFR-3 and lymph node metastasis in non-small cell lung cancer Correlation. Methods Fifty-two patients with non-small cell lung cancer (NSCLC), 196 lymph nodes, 10 lung tissue specimens from patients with benign lung diseases and 8 lymph nodes were collected. The expression of VEGFR-3 mRNA and protein in lung tissues and lymph node tissues were detected by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry respectively, and the lymphatic vessel density was measured. Results The expression of VEGFR-3 mRNA in lymph node metastasis-positive group was lower than that in lymph node-negative group (P <0.05), but not in lymph node metastasis-negative group There was no significant difference between the two groups (P> 0.05). There was no significant difference in the expression of VEGFR-3 mRNA between the positive lymph node and the negative lymph node in the positive lymph node metastasis group (P> 0.05). VEGFR-3 is not only expressed in lymphatic endothelial cells, but also expressed in the cytoplasm of small blood vessel endothelium and tumor cells. VEGFR-3 positive tube count in the cancer tissue was significantly higher than the cancer tissue in lymph node metastasis was significantly higher than the lymph node metastasis group. VEGFR-3 protein expression positive group compared with the negative group, lymphatic vessel density and lymph node metastasis were significantly increased. Conclusion The expression of VEGFR-3 mRNA and protein is significantly correlated with lymph node metastasis in lung cancer. It is important in predicting lymph node metastasis, improving molecular staging and guiding clinical treatment of lung cancer. Lymphatic vessel density is the key factor of tumor lymphatic metastasis, which indicates the occurrence of lymphangiogenesis and lymph node metastasis, which can be used as an indicator of tumor monitoring and prognosis.