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Loss of variety resistance to stripe rust (Puccinia striiformis Westend f. sp.tritici) is an important factor causing massiveperiodical epidemic of rust in wheat production. Creation and development of new races of rust pathogen have led toserious crisis of resistance loss in widely planted varieties. This has quickened the search for new resistance resources.Molecular marker could facilitate the identification of the location of novel genes. A line A-3 with high resistance(immune) to currently epidemic yellow rust races (CY29, 31, 32) was screened out in offspring of Triticum aestivum ×Thinopyrum ponticum. Segregation in F2 and BC1 populations indicated that the resistance was controlled by twoindependent genes: one dominant and one recessive. SSR markers were employed to map the two resistant genes in theF2 and BC1 populations. A marker WMC477-167 bp located on 2BS was linked to the dominant gene with genetic distanceof 0.4 cM. Another marker WMC364-208 bp located on 7BS was linked to the recessive-resistant gene with genetic distanceof 5.8 cM. The two genes identified in this paper might be two novel stripe rust resistant genes, which were temporarilydesignated as YrTp1 and YrTp2, respectively. The tightly linking markers facilitate transfer of the two resistant genes intothe new varieties to control epidemic of yellow rust.