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以落叶松转录组测序数据为基础,利用RT-PCR从落叶松中分离出一个1590 bp的APETALA2-Like转录因子基因全长编码序列,命名为La AP2L1。蛋白质特性分析显示La AP2L1编码529个氨基酸,为亲水性蛋白,相对分子质量为58.327 k D,等电点为6.45。二级结构主要由α-螺旋(alpha helix)、β-折叠(strand)和环肽链(loop)组成。多重序列比对和系统进化分析表明La AP2L1所编码的氨基酸与黑松、云杉等亲缘关系最近。同时,通过构建植物表达载体,利用浸花法转化模式植物拟南芥的功能研究发现,与转空载体对照拟南芥相比,过量表达La AP2L1的转基因拟南芥的叶片、茎、花和株高都显著增大、增高,表明落叶松La AP2L1转录因子可能参与了植物器官发育的调节。
Based on the sequencing data of larch transcriptome, a full-length coding sequence of 1590 bp APETALA2-Like transcription factor gene was isolated from larch by RT-PCR and named La AP2L1. Protein characterization showed that La AP2L1 encoded 529 amino acids, which was a hydrophilic protein with a relative molecular mass of 58.327 kD and an isoelectric point of 6.45. The secondary structure consists mainly of alpha helix, beta-strands and loops. Multiple sequence alignment and phylogenetic analysis indicated that the amino acids encoded by La AP2L1 were the closest to black pine and spruce. At the same time, we found that compared with Arabidopsis thaliana transfected with empty vector, La AP2L1 overexpression leaves, stems and flowers of Arabidopsis thaliana The plant height was significantly increased and increased, indicating that la AP2L1 transcription factor may be involved in the regulation of plant development.