根据葡萄卷叶伴随病毒 美国分离物的 GLRa V- 3CP基因序列 ,设计并合成了 1对该病毒的 PCR引物 ,利用 IC- RT- PCR成功地检查到合适大小的 PCR产物 ;同时将PCR产物克隆到中间载体 PGEM- 3Zf上 ,转化大肠杆菌 DH5α菌株 ,筛选阳性克隆。通过双酶切以及序列分析表明 ,扩增样品与美国分离物的同源性为 94.1 %。 According to the GLRa V-3CP gene sequence of Grapevine leafroll-associated virus US isolates, one pair of PCR primers for the virus was designed and synthesized. The PCR product of the appropriate size was successfully detected by IC-RT-PCR. At the same time, the PCR product was cloned To the intermediate vector PGEM- 3Zf, E. coli DH5α strain was transformed, screening positive clones. Double enzyme digestion and sequence analysis showed that the amplified samples had 94.1% homology with the American isolates.