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目的:探讨新疆哈萨克族体质指数(BMI)增高、高血压及血脂异常与β2-肾上腺素能受体(ADRB2)基因变异及非遗传因素的关系。方法:选取新疆和丰县牧区30～70岁哈萨克牧民671例(男283例,女388例),检测血清TC,TG,HDL,LDL。用PCR-限制性酶切片段多态性技术确定ADRB2基因Gln27Glu和Thr164Ile位点基因型。结果:①ADRB2基因Thr164Ile位点仅检测到5例Thr/Ile杂合子,约占1%,Ile/Ile基因型未检测到。②校正性别和年龄后,Gln27Glu和Thr164Ile位点各自不同基因型间BMI、血压和血脂平均水平比较差异均无统计学意义。③多元Logistic回归分析提示:TG、舒张压(DBP)是BMI增高的风险因素,与(Glu27Glu+吸烟)相比,(Gln27Glu+不吸烟)引起BMI增高风险增加1.69倍(95%CI:0.99～2.88),而(Gln27Gln+不吸烟)增高2.71倍(95%CI:1.49～4.92)。④多元方差分析提示,年龄、吸烟和饮酒对BMI、血压和TG的影响差异有统计学意义,而Gln27Glu位点仅对BMI有影响。⑤与体重正常者相比,超重者DBP增高的风险增加1.47倍(95%CI:1.03～2.09),肥胖者增加2.64倍(95%CI:1.69～4.11)。超重和肥胖者高TG的风险分别增加2.05倍(95%CI:1.27～3.31)和3.49倍(95%CI:2.06～5.92)。结论:新疆哈萨克族人群BMI和DBP,TG互为风险因素,且可能受到年龄、ADRB基因Glu27Glu变异和吸烟交互作用以及饮酒的影响。
Objective: To explore the relationship between the increase of body mass index (BMI), hypertension and dyslipidemia and genetic variation of β2-adrenergic receptor (ADRB2) and non-genetic factors in Kazak in Xinjiang. Methods: A total of 671 Kazakh herds aged 30-70 years (283 males and 388 females) from Xinjiang pastoral area were selected for detection of serum TC, TG, HDL and LDL. The genotypes of Gln27Glu and Thr164Ile in ADRB2 gene were determined by PCR-restriction fragment polymorphism. Results: ① Only 5 Thr / Ile heterozygotes were detected in the Thr164Ile site of ADRB2 gene, accounting for about 1%. The Ile / Ile genotype was not detected. ② After adjusting for gender and age, there was no significant difference in mean BMI, blood pressure and blood lipid between different genotypes of Gln27Glu and Thr164Ile loci. Multiple logistic regression analysis showed that TG and DBP were risk factors for increased BMI. Compared with Glu27Glu + smoking, Gln27Glu + non-smoking increased the risk of BMI by 1.69 times (95% CI: 0.99-2.88) (Gln27Gln + no smoking) increased by 2.71 times (95% CI: 1.49 ~ 4.92). Multivariate analysis of variance showed that there were significant differences in the effects of age, smoking and drinking on BMI, blood pressure and TG, while Gln27Glu only affected BMI. ⑤ The risk of DBP increase in overweight patients was 1.47 times (95% CI: 1.03-2.09) and 2.64 times (95% CI: 1.69-4.11) in obese subjects compared with normal weight subjects. The risk of high TG in overweight and obese individuals increased 2.05-fold (95% CI: 1.27-3.31) and 3.49-fold (95% CI 2.06-5.92), respectively. Conclusion: The BMI and DBP, TG of Xinjiang Kazak population are risk factors for each other and may be affected by age, Glu27Glu mutation of ADRB gene, smoking interaction and alcohol consumption.