病理会诊往往只有1张HE切片,较难处理,常需借助特殊染色予以诊断。10余年来,我们采用同一切片上分段染色,有时达3种以上,取得良好效果。为便于推广这一方法,特介绍如下。选材凡石蜡切片皆适用。方法用钻石笔割断盖玻片,在酒精灯上略加温,小心推开盖玻片,使需染部分予以暴露。在二甲苯中轻轻洗去树脂胶,时间不宜过长,以刚刚洗 Pathological consultations often have only one HE slice, which is difficult to handle and often requires special staining for diagnosis. For more than 10 years, we have used the same section on the segmented staining, sometimes up to 3 or more, and achieved good results. In order to facilitate the promotion of this method, it is specifically introduced as follows. All paraffin sections are suitable for selection. METHODS: The coverslip was cut with a diamond pen, slightly warmed on an alcohol lamp, and the cover glass was carefully pushed open so that the part to be dyed was exposed. Lightly wash away the resin glue in xylene, the time should not be too long, just wash