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目的研究长白山珍稀药材山兰Oreorchas patens假鳞茎醋酸乙酯部位的化学成分。方法采用硅胶柱色谱、薄层色谱、重结晶、Sephadex LH-20凝胶柱等方法进行分离纯化,通过理化性质及多种波谱数据(~1H-NMR、~(13)C-NMR)鉴定化合物的结构。结果从山兰假鳞茎醋酸乙酯部位分离出11个化合物,分别鉴定为β-谷甾醇(1)、β-胡萝卜苷(2)、邻苯二甲酸二(2-乙基己)酯(3)、邻苯二甲酸-1-丁酯-2-异丁酯(4)、邻苯二甲酸二戊酯(5)、β-谷甾醇亚油酸酯(6)、邻苯二甲酰正二丁酯(7)、对苯二甲酸二丁酯(8)、卷瓣兰蒽(9)、二十五烷(10)、三十六烷(11)。结论化合物3~11均为首次从该属植物中分离得到。
Objective To study the chemical constituents of the pseudobulbular ethyl acetate fraction of Oreorchas patens, a rare medicinal herb from Changbai Mountain. Methods The compounds were isolated by silica gel column chromatography, TLC, recrystallization and Sephadex LH-20 gel column chromatography. The compounds were identified by physicochemical properties and spectroscopic data (~ 1H-NMR and ~ (13) C-NMR) Structure. Results Eleven compounds were isolated from the ethyl acetate fraction of P. annua and identified as β-sitosterol (1), β-daucosterol (2), di-2-ethylhexyl phthalate (4), dipentyl phthalate (5), β-sitosterol linoleate (6), phthalic acid di-n-butyl phthalate Butyl phthalate (7), dibutyl terephthalate (8), coumarin anthracene (9), pentacosane (10), and hexacosane (11). Conclusion Compounds 3 ~ 11 were isolated from this genus for the first time.