论文部分内容阅读
本文作者用同位素标记法来探索选择性掺入麻风杆菌的核苷,以便用于筛选抗麻风药物,测定药物敏感性及耐药性.选择27例经联合化疗的多菌的瘤型病人为研究对象,常规切割或6mm钻孔取活检组织,无菌条件下匀浆化,制备麻风杆菌提取物并计数.所有麻风杆菌提取物均作了无污染检查.用腹腔灌洗法得到BALB/C小鼠腹腔巨噬细胞,在96孔培养板上培养,以新鲜麻风杆菌感染巨噬细胞,再以含各种放射性同位素标记物(1μCi/m嘌呤和嘧啶核苷)的培养基培养.在测试药物对麻风杆菌的抑制作用时,用RPMll640将药物稀释成不同浓度,与放射性标记物一起加入.在特定的时间收集培养物,用β-闪烁计数器计数.作者还将无细胞的单纯培养基与含巨噬细胞的培养基进行比
The authors used isotope labeling to explore the selective incorporation of leprosy nucleosides for the screening of anti-leprosy drugs for the determination of drug sensitivity and drug resistance.Selecting 27 cases of combined chemotherapy of multi-bacteria tumor patients for the study Objects, conventional cutting or 6mm drilling biopsy tissue, homogenized under sterile conditions, preparation of leprosy extract and count all leprosy extract were made non-polluting examination. BALB / C small peritoneal lavage obtained Mouse peritoneal macrophages were cultured in 96-well plates, macrophages were infected with fresh M. leprae and cultured in a medium containing various radioactive isotope labels (1 μCi / m purine and pyrimidine nucleoside) For inhibition of M. leprae, the drug was diluted to varying concentrations with RPMll640 and added with radiolabel. Cultures were harvested at specific times and counted with a [beta] -scintillation counter. The authors also compared cell free medium with The ratio of macrophages in culture medium