【摘 要】
：
目的:建立不同种源黄花蒿内青蒿素及青蒿乙素含量测定的方法,比较不同来源黄花蒿种质在冰培均一化生长条件下青蒿素与青蒿乙素含量的差异,分析影响黄花蒿主要成分含量差异的关键因素.方法:黄花蒿种子采用随机排列水培混合培养,运用超高效液相色谱-串联质谱法,ACQUITY UPLC(R) BEHC18色谱柱(2.1 mm×100 mm,1.7 μm),流动相选择水-乙腈(95:5,含0.1％甲酸,A)-乙腈-水(95:5,含0.1％甲酸,B)进行梯度洗脱(0～3.5 min,25％～1％A;3.5～3.6 min,1
【机 构】
：
中国中医科学院中药研究所,北京100700;成都中医药大学,成都611137;中国医学科学院·北京协和医学院药用植物研究所,北京100193;大理大学,云南大理671003;山东中医药大学,济南250
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目的:建立不同种源黄花蒿内青蒿素及青蒿乙素含量测定的方法,比较不同来源黄花蒿种质在冰培均一化生长条件下青蒿素与青蒿乙素含量的差异,分析影响黄花蒿主要成分含量差异的关键因素.方法:黄花蒿种子采用随机排列水培混合培养,运用超高效液相色谱-串联质谱法,ACQUITY UPLC(R) BEHC18色谱柱(2.1 mm×100 mm,1.7 μm),流动相选择水-乙腈(95:5,含0.1％甲酸,A)-乙腈-水(95:5,含0.1％甲酸,B)进行梯度洗脱(0～3.5 min,25％～1％A;3.5～3.6 min,1％～25％A;3.6～5.0 min,25％A),流速0.4 mL· min-1,电喷雾离子源,正离子模式,检测不同种源黄花蒿中青蒿素及青蒿乙素的含量差异.结果:所建立的方法灵敏度高、分离度良好.不同种源黄花蒿在相同培养条件下,即25 ℃恒温下循环水培养,青蒿素和青蒿乙素含量存在较大差异,其中青蒿素含量较高的黄花蒿种源产地是云南,质量分数达3810.597 μg·g-1;青蒿乙素含量较高的黄花蒿种源地是山西,质量分数1691.747μg·g-1,青蒿素含量按照从高到低种源地排列依次为云南＞海南＞湖北＞广西＞浙江＞山西＞北京＞山东＞黑龙江＞甘肃.相关性分析发现,以地域划分时,青蒿素含量与黄花蒿来源地的纬度呈显著负相关,但青蒿素与青蒿乙素含量均与经度无显著相关性.结论:同一培养环境不同种源黄花蒿内青蒿素及青蒿乙素含量存在显著差异,影响黄花蒿中青蒿素及青蒿乙素含量的主导因素可能是其遗传背景,提示改良青蒿品种是后续黄花蒿栽培中提升质量的关键因素.
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