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目的:建立测定透骨香中槲皮苷含量的高效液相色谱法。方法:采用Benetnach C18(4.6mm×15mm,5μm)填料色谱柱,流动相为0.01mol.L-1磷酸二氢钾-甲醇-冰醋酸(体积比65∶35∶0.7),流速1.0mL.min-1,检测波长为254nm,柱温30℃。结果:槲皮苷在(0.044～0.154)μg范围内与峰面积线性关系良好(r=0.999 3),平均加样回收率为101.25%,RSD为1.92%。结论:该方法快速、简便,专属性强,重现性好,准确度高,可用于透骨香中槲皮苷含量的测定。
Objective: To establish a high performance liquid chromatographic method for the determination of quercetin in Methods: Benetnach C18 (4.6mm × 15mm, 5μm) packed column was used, the mobile phase consisted of 0.01mol·L-1 potassium dihydrogen phosphate-methanol-glacial acetic acid (volume ratio 65:35:0.7), the flow rate was 1.0mL · min -1, detection wavelength of 254nm, column temperature 30 ℃. Results: Quercetin showed good linearity (r = 0.999 3) in the range of (0.044 ~ 0.154) μg with an average recovery of 101.25% and RSD of 1.92%. Conclusion: The method is rapid, simple, specific, reproducible and accurate. It can be used for the determination of quercetin in